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The SEM is inherently parfocal - the image stays in focus with changes in magnification. This animation shows a valve of the diatom Pseudo-nitzschia seriata cycled through magnifications ranging from x1,200 to x12,000. Once focused at a higher magnification, the image will remain in focus at any lower magnification.

P. seriata zoom

Image courtesy Dr. Stephen Bates, DFO Moncton (emeritus)